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Antikörper

Isovaleryl-CoA dehydrogenase/IVD Monoclonal Antibody

Produktgrößen
100 ul
£346,00
AN200143P-100UL
Über dieses Produkt
SKU:
AN200143P
Zusätzliche Namen:
ACAD,IVD,ACAD2,FLJ12715,FLJ34849,Isovaleryl CoA dehydrogenase,Isovaleryl Coenzyme A dehydrogenase,Isovaleryl-CoA dehydrogenase,mitochondrial
Anwendung:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS
translate.label.attr.clone:
A991
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
Isovaleryl-CoA dehydrogenase (IVD) is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. The genetic deficiency of IVD results in an accumulation of isovaleric acid, which is toxic to the central nervous system and leads to isovaleric acidemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Host:
Mouse
Immunogen:
Recombinant Human Isovaleryl-CoA dehydrogenase / IVD protein
Isotyp:
IgG2b
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Monoclonal Antibody
Flow cytometric analysis of Human IVD expression on HeLa cells. The cells were stained with purified anti-Human IVD, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human IVD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human IVD Monoclonal Antibody (1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.
Immunoprecipitation analysis using 0.5 uL anti-IVD mouse Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using IVD mouse Monoclonal Antibody at a dilution of 1:500. Lane A:0.5 mg Jurkat Whole Cell Lysate, Lane B:0.5 mg A549 Whole Cell Lysate
Western Blot with Isovaleryl-CoA dehydrogenase / IVD Monoclonal Antibody at dilution of 1:500. Lane A: Jurkat Whole Cell Lysate, Lane B: Hela Whole Cell Lysate, Lysates/proteins at 30 ug per lane.