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Antikörper

PGDH/PHGDH Monoclonal Antibody

Produktgrößen
100 ul
£346,00
AN200135P-100UL
Über dieses Produkt
SKU:
AN200135P
Zusätzliche Namen:
HEL-S,PHGDH,3-PGDH,3PGDH,HEL-S-113,NLS,NLS1,PDG,PGAD,PGD,PGDH,PHGDHD,SERA,PGDH3
Anwendung:
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Buffer:
PBS
translate.label.attr.clone:
A983
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.
Host:
Mouse
Immunogen:
Recombinant Human PGDH / PHGDH protein
Isotyp:
IgG2b
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Monoclonal Antibody
Flow cytometric analysis of Human PHGDH expression on HeLa cells. The cells were stained with purified anti-Human PHGDH, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human PHGDH in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human PHGDH Monoclonal Antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red). Positive staining was localized to cytoplasm.