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Antikörper

4E-BP1/EIF4EBP1 Monoclonal Antibody

Produktgrößen
100 ul
£346,00
AN200088P-100UL
Über dieses Produkt
SKU:
AN200088P
Zusätzliche Namen:
4EBP;eIF4E binding protein;eukaryotic translation initiation factor 4E-binding protein;4E-BP;EIF4EBP;EIF4EBP1;4E-BP1;4EBP1;BP-1;PHAS-I;eukaryotic translation initiation factor 4E-binding protein 1;BP 1;eIF4E binding protein 1;eIF4E-binding protein 1;PHASI;Phosphorylated heat- and acid-stable protein regulated by insulin 1;4EBP1;4E-BP1;BP-1;eukaryotic translation initiation factor 4E-binding protein 1;PHAS-I
Anwendung:
Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
A936
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Host:
Mouse
Immunogen:
Recombinant Human 4E-BP1 / EIF4EBP1 Protein
Isotyp:
IgG1
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Monoclonal Antibody
Immunofluorescence analysis of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human 4EBP1 Monoclonal Antibody (1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to nucleus and cytoplasm.
Western Blot with 4E-BP1 / EIF4EBP1 Monoclonal Antibody at dilution of 1:500. Lane A: A431 Whole Cell Lysate, Lane B: K562 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.