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Antikörper

CREB1 Monoclonal Antibody

Produktgrößen
100 ul
£346,00
AN200060P-100UL
Über dieses Produkt
SKU:
AN200060P
Zusätzliche Namen:
CREB1;CREB;CREB-1;Active transcription factor CREB;cAMP response element binding protein;cAMP response element binding protein 1;cAMP responsive element binding protein 1;cAMP-responsive element-binding protein 1;Cyclic AMP-responsive element-binding protein 1;MGC9284;OTTHUMP00000163864;OTTHUMP00000163865;OTTHUMP00000206660;OTTHUMP00000206662;OTTHUMP00000206667;Transactivator protein
Anwendung:
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
A908
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
Host:
Mouse
Immunogen:
Recombinant Human CREB1 Protein
Isotyp:
IgG2b
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Monoclonal Antibody
Flow cytometric analysis of Human CREB1 expression on HepG2 cells. The cells were stained with purified anti-Human CREB1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human CREB1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CREB1 Monoclonal Antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to nucleus.