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Antikörper

ALDH1/ALDH1A1/ALDC Monoclonal Antibody

Produktgrößen
20 ul
£189,00
AN200041P-20UL
100 ul
£346,00
AN200041P-100UL
Über dieses Produkt
SKU:
AN200041P
Zusätzliche Namen:
Retinal Dehydrogenase;Aldehyde Dehydrogenase Family 1 Member A;ALDH1A;ALDH-E;HEL;ALDH1A1;ALDC;ALDH-E1;ALDH1;ALDH11;HEL-9;HEL-S-53e;HEL12;PUMB1;RALDH1;Aldehyde Dehydrogenase Cytosolic;Aldehyde Dehydrogenase Family 1 Member A1;ALHDII;PUMB10;RALDH 1;Retinal Dehydrogenase 1
Anwendung:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
A885
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.
Host:
Mouse
Immunogen:
Recombinant Human ALDH1 protein
Isotyp:
IgG1
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Monoclonal Antibody
Flow cytometric analysis of Human ALDH1A1 on A549 cells. Cells were stained with purified anti-Human ALDH1A1, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human ALDH1A1 in MCF7 or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human ALDH1A1 Monoclonal Antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.
Western Blot with ALDH1 / ALDH1A1 / ALDC Monoclonal Antibody at dilution of 1:500. Lane A: HepG2 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.