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Antikörper

NME1/NDKA Monoclonal Antibody

Produktgrößen
100 ul
£346,00
AN200029N-100UL
Über dieses Produkt
SKU:
AN200029N
Zusätzliche Namen:
NME,Metastasis Inhibition Factor nm,NM23-H,NME1,AWD,GAAD,NB,NBS,NDKA,NDPK-A,NDPKA,NM23,NM23-H1,Granzyme A-Activated DNase,Metastasis Inhibition Factor nm23,NDK A,NDP Kinase A,Nucleoside Diphosphate Kinase A,Tumor Metastatic Process-Associated Protein,drosophila,Granzyme A activated DNase,GZMA activated DNase,homolog 1,homolog of,included,NM23 long variant,NM23H1B,NM23-M1,NME/NM23 nucleoside diphosphate kinase 1,NME1-NME2 spliced read-through transcript,Nonmetastatic cells 1,Non-metastatic cells 1,Nonmetastatic protein 23,protein (NM23A) expressed in,protein expressed in
Anwendung:
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Buffer:
0.2 um filtered solution in 20 mM MES, 100 mM NaCl, 10% Trehalose, 0.02% Tween 80, pH 6.0
translate.label.attr.clone:
A873
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME1), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
Host:
Mouse
Immunogen:
Recombinant Human NME1 / NDKA protein
Isotyp:
IgG1
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Monoclonal Antibody
Flow cytometric analysis of Human NME1 expression on HeLa cells. The cells were stained with purified anti-Human NME1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human NME1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human NME1 Monoclonal Antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.