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Antikörper

CD166/ALCAM Monoclonal Antibody

Produktgrößen
100 ul
£346,00
AN200008P-100UL
Über dieses Produkt
SKU:
AN200008P
Zusätzliche Namen:
Activated leukocyte cell adhesion molecule;CD166;CD166 antigen;MEMD;ALCAM
Anwendung:
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
A852
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
CD166, also known as the CD6 ligand or the Activated Leukocyte Cell Adhesion Molecule (ALCAM), is a 100-105 kD transmembrane glycoprotein. It belongs to the Ig superfamily of proteins and expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently CD166 has also been used as a potential cancer stem cell marker. The antibody reacts withHumanactivated leukocyte cell adhesion molecule (ALCAM).
Host:
Mouse
Immunogen:
Recombinant Human CD166 / ALCAM protein
Isotyp:
IgG1
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Monoclonal Antibody
Flow cytometric analysis of human ALCAM(CD166) expression on Jurkat cells. Cells were stained with purified anti-Human ALCAM(CD166), then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence staining of Human CD166 in MSC cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD166 Monoclonal Antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cells membrane.