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Antikörper

ICAM-1/CD54 Monoclonal Antibody

Produktgrößen
20 ul
£167,00
AN200003P-20UL
100 ul
£346,00
AN200003P-100UL
Über dieses Produkt
SKU:
AN200003P
Zusätzliche Namen:
P3.;MyD;Intercellular adhesion molecule;MALA;BB2;CD54;P3.58;ICAM1;MALA-2;MyD10;ICAM-1;Intercellular adhesion molecule 1;Major group rhinovirus receptor;Antigen identified by monoclonal BB2;BB 2;CD 54;CD_antigen=CD54;Cell surface glycoprotein P3.58;Human rhinovirus receptor;ICAM 1;intercellular adhesion molecule 1 (CD54);MALA 2;MALA2;MyD 10;Surface antigen of activated B cells
Anwendung:
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
A847
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
ICAM-1 is a member of the immunoglobulin superfamily whose expression is upregulated on leukocytes, epithelial cells and resting endothelial cells in response to inflammatory signals. ICAM-1 binds the leukocyte integrins LFA-1 and Mac-1.
Host:
Mouse
Immunogen:
Recombinant Human ICAM-1 protein
Isotyp:
IgG1
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Monoclonal Antibody
Flow cytometric analysis of Human ICAM1 (CD54) in HUVEC cells. HUVEC cells (Human umbilical vein endothelial cells) were either treated with TNF-A Alpha (50ng/mL,18 hours) (right panel) or untreated (left panel). The cells were harvested and stained with purified human ICAM-1, then a FITC-conjugated second step antibody.
Immunofluorescence staining of Human ICAM1 in MSC cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with Mouse anti-Human ICAM1 Monoclonal Antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cell membrane.