GC-1 spg Cells - Mouse cell lines
Produktgrößen
1 each
£755,00
300375-1EACH
Über dieses Produkt
- SKU:
- 300375
- Zusätzliche Namen:
- GC-1spg, GC-1, GC1-SPG
- Anwendung:
- Tissue Culture
- CE/IVD:
- Our cells are provided for in vitro laboratory research purposes exclusively and are not intended fo
- Weitere Details:
- The GC-1 spg cell line was immortalized through transfection with the pSV3-neo plasmid, which harbors the coding sequences for the SV40 large T antigen and neomycin resistance. This genetic modification not only provides resistance to certain antibiotics but also promotes the continuous growth of the cells by altering their cell cycle regulation, thus bypassing the Hayflick limit typical of primary cells. This process of immortalization allows the cells to maintain proliferative capacity while retaining key phenotypic characteristics of spermatogonia. Phenotypically, the GC-1 spg cell line exhibits characteristics that are indicative of a transitional stage between type B spermatogonia and primary spermatocytes, making it an especially relevant model for studying the early stages of spermatogenesis. The cells express two testis-specific isoproteins: cytochrome c and lactate dehydrogenase C4. These markers are crucial for studying cell metabolism and energy management during spermatogenesis, reflecting the unique metabolic pathways active in germ cells. The expression of these specific isoproteins underscores the cell line's utility in exploring the biochemical and physiological aspects of testicular cell function and development.
- Versandbedingungen:
- Dry Ice
- Lagerbedingungen:
- Liquid N2, -70[o]C Avoid freeze/thaw cycles.
- Hersteller:
- Cytion
- Typ:
- Cells: Cell Lines
- Datenblatt des Herstellers:6d38c8c53ab44ce38116e945f749951d