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Antikörper

KIFAP3 Recombinant Monoclonal Antibody

Produktgrößen
50 ul
£235,00
CSB-RA869779A0HU-50UL
100 ul
£392,00
CSB-RA869779A0HU-100UL
Über dieses Produkt
SKU:
CSB-RA869779A0HU
Zusätzliche Namen:
Kinesin-associated protein 3 (KAP-3) (KAP3) (Smg GDS-associated protein), KIFAP3, KIF3AP SMAP
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
28E9
Klonalität:
Recombinant Monoclonal
Immunogen:
A synthesized peptide derived from human KIFAP3
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Recombinant Antibodies
Immunofluorescence staining of PC-3 cell with CSB-RA869779A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 552-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-RA869779A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: KIFAP3 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 92, 87, 83 kDa Observed band size: 72-95 kDa