Skip to content

Warenkorb

Der Warenkorb ist leer.

Gesamt (exkl. MwSt.) £0,00
Warenkorb überprüfen & bezahlen
Antikörper

ALDOA Recombinant Monoclonal Antibody

Produktgrößen
50 ul
£235,00
CSB-RA571479A0HU-50UL
100 ul
£392,00
CSB-RA571479A0HU-100UL
Über dieses Produkt
SKU:
CSB-RA571479A0HU
Zusätzliche Namen:
Fructose-bisphosphate aldolase A (EC 4.1.2.13) (Lung cancer antigen NY-LU-1) (Muscle-type aldolase), ALDOA, ALDA
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
7D8
Klonalität:
Recombinant Monoclonal
Weitere Details:
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).
Immunogen:
A synthesized peptide derived from human Aldolase
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human, Mouse, Rat
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Recombinant Antibodies
Immunofluorescence staining of Hela Cells with CSB-RA571479A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
IHC image of CSB-RA571479A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, Mouse Liver whole cell lysate, Rat Liver whole cell lysate All lanes: Aldolase antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 46 kDa Observed band size: 40 kDa