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Antikörper

FUBP1 Recombinant Monoclonal Antibody

Produktgrößen
50 ul
£235,00
CSB-RA157765A0HU-50UL
100 ul
£392,00
CSB-RA157765A0HU-100UL
Über dieses Produkt
SKU:
CSB-RA157765A0HU
Zusätzliche Namen:
Far upstream element-binding protein 1 (FBP) (FUSE-binding protein 1) (DNA helicase V) (hDH V), FUBP1
Anwendung:
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
7C3
Klonalität:
Recombinant Monoclonal
Weitere Details:
Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.
Immunogen:
A synthesized peptide derived from human FUBP1
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Recombinant Antibodies
Overlay histogram showing Jurkat cells stained with CSB-RA157765A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of Hela Cells with CSB-RA157765A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
IHC image of CSB-RA157765A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA157765A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating FUBP1 in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA157765A0HU in Jurkat whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA157765A0HU(2ug)+ Jurkat whole cell lysate(500ug) Lane 3: Jurkat whole cell lysate (10ug)