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Antikörper

MIF Recombinant Monoclonal Antibody

Produktgrößen
50 ul
£235,00
CSB-RA013826MA1HU-50UL
100 ul
£392,00
CSB-RA013826MA1HU-100UL
Über dieses Produkt
SKU:
CSB-RA013826MA1HU
Zusätzliche Namen:
GIF antibody; GLIF antibody; Glycosylation inhibiting factor antibody; Glycosylation-inhibiting factor antibody; L-dopachrome isomerase antibody; L-dopachrome tautomerase antibody; Macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody; Macrophage migration inhibitory factor antibody; MIF antibody; MIF protein antibody; MIF_HUMAN antibody; MMIF antibody; Phenylpyruvate tautomerase antibody
Anwendung:
ELISA, Flow Cytometry, Immunofluorescence
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
6E11
Klonalität:
Recombinant Monoclonal
Immunogen:
Recombinant Human MIF protein
Isotyp:
IgG1
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Recombinant Antibodies
Overlay Peak curve showing PC3 cells stained with CSB-RA013826MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of hela cell with CSB-RA013826MA1HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).
Immunofluorescence staining of U251 cell with CSB-RA013826MA1HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).