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Antikörper

Acetyl-Histone H3.1 (K14) Recombinant Monoclonal Antibody

Produktgrößen
50 ul
£235,00
CSB-RA010418A14ACHU-50UL
100 ul
£392,00
CSB-RA010418A14ACHU-100UL
Über dieses Produkt
SKU:
CSB-RA010418A14ACHU
Zusätzliche Namen:
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA, AND, HIST1H3B, H3FL, AND, HIST1H3C, H3FC, AND, HIST1H3D, H3FB, AND, HIST1H3E, H3FD, AND, HIST1H3F, H3FI, AND, HIST1H3G, H3FH, AND, HIST1H3H, H3FK, AND, HIST1H3I, H3FF, AND, HIST1H3J, H3FJ
Anwendung:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
3H3
Klonalität:
Recombinant Monoclonal
Weitere Details:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Immunogen:
A synthesized peptide
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human, Rat
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Recombinant Antibodies
Immunocytochemistry analysis of CSB-RA010418A14acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010418A14acHU at 1:46,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Western Blot Positive WB detected in Hela whole cell lysateepG2 whole cell lysateH-SY5Y whole cell lysateat brain tissue All lanes Acetyl-Histone H3.1(K14)antibody at 0.75ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa