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Antikörper

GZMB Recombinant Monoclonal Antibody

Produktgrößen
50 ul
£235,00
CSB-RA010082MA1HU-50UL
100 ul
£392,00
CSB-RA010082MA1HU-100UL
Über dieses Produkt
SKU:
CSB-RA010082MA1HU
Zusätzliche Namen:
C11 antibody; Cathepsin G like 1 antibody; Cathepsin G-like 1 antibody; CCPI antibody; CGL 1 antibody; CGL1 antibody; CSP B antibody; CSPB antibody; CTLA 1 antibody; CTLA-1 antibody; CTLA1 antibody; CTSGL1 antibody; Cytotoxic serine protease B antibody; Cytotoxic T lymphocyte associated serine esterase 1 antibody; Cytotoxic T lymphocyte proteinase 2 antibody; Cytotoxic T-lymphocyte proteinase 2 antibody; Fragmentin 2 antibody; Fragmentin-2 antibody; GRAB_HUMAN antibody; Granzyme 2 antibody; Granzyme B (granzyme 2; cytotoxic T lymphocyte associated serine esterase 1) antibody; Granzyme B antibody; Granzyme-2 antibody; GranzymeB antibody; GRB antibody; Gzmb antibody; Hlp antibody; Human lymphocyte protein antibody; Lymphocyte protease antibody; Protease; serine; B antibody; SECT antibody; T cell serine protease 1 3E antibody; T cell serine protease 1-3E antibody; T-cell serine protease 1-3E antibody
Anwendung:
ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
9B2
Klonalität:
Recombinant Monoclonal
Immunogen:
Recombinant Human GZMB protein
Isotyp:
IgG2a
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Recombinant Antibodies
Overlay Peak curve showing THP-1 cells stained with CSB-RA010082MA1HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was mouse IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
IHC image of CSB-RA010082MA1HU diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in:K562 whole cell lysate (20ug) All lanes: GZMB antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size:27.7 kDa Observed band size:28 kDa Exposure time:300s