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Antikörper

GDNF Recombinant Monoclonal Antibody

Produktgrößen
50 ul
£235,00
CSB-RA009356A0HU-50UL
100 ul
£392,00
CSB-RA009356A0HU-100UL
Über dieses Produkt
SKU:
CSB-RA009356A0HU
Zusätzliche Namen:
Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF
Anwendung:
ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
phosphate buffered saline
translate.label.attr.clone:
11D2
Klonalität:
Recombinant Monoclonal
Weitere Details:
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Immunogen:
A synthesized peptide derived from human GDNF
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Recombinant Antibodies
Overlay histogram showing SH-SY5Y cells stained with CSB-RA009356A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
IHC image of CSB-RA009356A0HU diluted at 1:107.5 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA009356A0HU diluted at 1:107.5 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating GDNF in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA009356A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA009356A0HU (3ug) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (20ug)
Western Blot Positive WB detected in: HepG2 whole cell lysate, 293T whole cell lysate, SH-SY5Y whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate All lanes: GDNF antibody at 1ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 24, 21, 26, 23, 19 KDa Observed band size: 24 KDa