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Antikörper

Phospho-CREB1 (S133) Recombinant Monoclonal Antibody

Produktgrößen
50 ul
£235,00
CSB-RA005947A133PHHU-50UL
100 ul
£392,00
CSB-RA005947A133PHHU-100UL
Über dieses Produkt
SKU:
CSB-RA005947A133PHHU
Zusätzliche Namen:
Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline
translate.label.attr.clone:
3C11
Klonalität:
Recombinant Monoclonal
Weitere Details:
Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.
Immunogen:
A synthesized peptide derived from human Phospho-CREB1 (S133)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Recombinant Antibodies
Immunofluorescence staining of Hela cells with CSB-RA005947A133phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
IHC image of CSB-RA005947A133phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA005947A133phHU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in Hela whole cell lysate,293 whole cell lysate All lanes Phospho-CREB1 antibody at 1.65ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 46 KDa Observed band size: 46 KDa