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Antikörper

CD147 Recombinant Monoclonal Antibody

Produktgrößen
50 ul
£235,00
CSB-RA002831A0HU-50UL
100 ul
£392,00
CSB-RA002831A0HU-100UL
Über dieses Produkt
SKU:
CSB-RA002831A0HU
Zusätzliche Namen:
5A11 antigen antibody; 5F7 antibody; BASI_HUMAN antibody; Basigin (Ok blood group) antibody; Basigin antibody; Blood brain barrier HT7 antigen antibody; Bsg antibody; CD 147 antibody; CD147 antibody; CD147 antigen antibody; Collagenase stimulatory factor antibody; EMMPRIN antibody; Extracellular matrix metalloproteinase inducer antibody; Leukocyte activation antigen M6 antibody; M 6 antibody; M6 antibody; M6 leukocyte activation antigen antibody; Neurothelin antibody; OK antibody; OK blood group antibody; OK blood group antigen antibody; TCSF antibody; Tumor cell derived collagenase stimulatory factor antibody; Tumor cell-derived collagenase stimulatory factor antibody
Anwendung:
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
11F3
Klonalität:
Recombinant Monoclonal
Immunogen:
Recombinant Human CD147 protein
Isotyp:
IgG2a
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Recombinant Antibodies
The Binding Activity of CD147 with Anti-CD147 recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD147 (CSB-MP002831HU1) at 2 ug/ml can bind Anti-CD147 recombinant Antibody, the EC50 is 21.95-33.12 ng/ml.
Immunofluorescence staining of Hela cells with CSB-RA002831A0HU at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
IHC image of CSB-RA002831A0HU diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA002831A0HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA002831A0HU diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.