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Antikörper

ATP7B Recombinant Monoclonal Antibody

Produktgrößen
50 ul
£235,00
CSB-RA002415MA1HU-50UL
100 ul
£392,00
CSB-RA002415MA1HU-100UL
Über dieses Produkt
SKU:
CSB-RA002415MA1HU
Zusätzliche Namen:
ATP7B antibody; ATP7B_HUMAN antibody; ATPase, Cu(2+) transporting, beta polypeptide antibody; ATPase, Cu++ transporting, beta polypeptide antibody; Copper pump 2 antibody; Copper transporting ATPase 2 antibody; PWD antibody; Toxic milk antibody; tx antibody; WC1 antibody; WD antibody; Wilson disease associated protein antibody; Wilson disease-associated protein antibody; WND antibody; WND/140 kDa antibody
Anwendung:
ELISA, Flow Cytometry, Immunofluorescence
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
24A4
Klonalität:
Recombinant Monoclonal
Immunogen:
Recombinant Human ATP7B protein
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Recombinant Antibodies
Overlay Peak curve showing Hela cells stained with CSB-RA002415MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of Hela cell with CSB-RA002415MA1HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).