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Antikörper

ACTA1 Recombinant Monoclonal Antibody

Produktgrößen
50 ul
£235,00
CSB-RA001205A0HU-50UL
100 ul
£392,00
CSB-RA001205A0HU-100UL
Über dieses Produkt
SKU:
CSB-RA001205A0HU
Zusätzliche Namen:
Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA
Anwendung:
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
25E3
Klonalität:
Recombinant Monoclonal
Weitere Details:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Immunogen:
A synthesized peptide
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Recombinant Antibodies
Overlay histogram showing Hela cells stained with CSB-RA001205A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of HepG2 cells with CSB-RA001205A0HU at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
IHC image of CSB-RA001205A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Raw264.7 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat heart tissue All lanes: Actin antibody at 0.95ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa