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Antikörper

MAGED2 Antibody

Produktgrößen
50 ug
£186,00
CSB-PA891976LA01HU-50UG
100 ug
£334,00
CSB-PA891976LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA891976LA01HU
Zusätzliche Namen:
11B6 antibody; BCG 1 antibody; BCG-1 antibody; Breast cancer-associated gene 1 protein antibody; HCA10 antibody; Hepatocellular carcinoma-associated protein JCL-1 antibody; JCL 1 antibody; MAGD2_HUMAN antibody; MAGE D antibody; MAGE D2 antigen antibody; MAGE-D2 antigen antibody; MAGED2 antibody; Melanoma antigen family D 2 antibody; Melanoma associated antigen D2 antibody; Melanoma-associated antigen D2 antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Klonalität:
Polyclonal
Weitere Details:
Regulates the expression, localization to the plasma membrane and function of the sodium chloride cotransporters SLC12A1 and SLC12A3, two key components of salt reabsorption in the distal renal tubule.
Host:
Rabbit
Immunogen:
Recombinant Human Melanoma-associated antigen D2 protein (38-285AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA891976LA01HU at 1:145, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA891976LA01HU diluted at 1:320 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA891976LA01HU diluted at 1:320 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating MAGED2 in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA891976LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA891976LA01HU (6ug) + HepG2 whole cell lysate (500ug) Lane 3: HepG2 whole cell lysate (20ug)
Western Blot Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate All lanes: MAGED2 antibody at 4.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65, 64 kDa Observed band size: 65, 64 kDa