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Antikörper

GJD2 Antibody

Produktgrößen
50 ug
£186,00
CSB-PA891536LA01HU-50UG
100 ug
£334,00
CSB-PA891536LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA891536LA01HU
Zusätzliche Namen:
Connexin 36 / GJA9 antibody; Connexin 36 antibody; Connexin-36 antibody; Cx36 antibody; CXD2_HUMAN antibody; Gap junction alpha-9 protein antibody; Gap junction delta-2 protein antibody; Gap junction protein alpha 9 antibody; gap junction protein; alpha 9; 36kDa antibody; GJA9 antibody; GJD2 antibody; MGC138315 antibody; MGC138319 antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Host:
Rabbit
Immunogen:
Recombinant Human Gap junction delta-2 protein (99-197AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
IHC image of CSB-PA891536LA01HU diluted at 1:500 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA891536LA01HU diluted at 1:500 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: U87 whole cell lysate All lanes: GJD2 antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa
Immunofluorescence staining of Hela cells with CSB-PA891536LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).