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Antikörper

HECW2 Antibody

Produktgrößen
50 ug
£186,00
CSB-PA889188LA01HU-50UG
100 ug
£334,00
CSB-PA889188LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA889188LA01HU
Zusätzliche Namen:
C2 and WW domain-containing protein 2 antibody; E3 ubiquitin protein ligase HECW2 antibody; E3 ubiquitin-protein ligase HECW2 antibody; HECT antibody; HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 antibody; Hecw2 antibody; HECW2_HUMAN antibody; KIAA130 antibody; KIAA1301 antibody; NEDD4 like E3 ubiquitin protein ligase 2 antibody; NEDD4 Related E3 Ubiquitin Ligase antibody; NEDD4-like E3 ubiquitin-protein ligase 2 antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73 (PubMed:12890487). Involved in the regulation of mitotic metaphase/anaphase transition (PubMed:24163370).
Host:
Rabbit
Immunogen:
Recombinant Human E3 ubiquitin-protein ligase HECW2 protein (495-641AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of A549 cells with CSB-PA889188LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA889188LA01HU diluted at 1:500 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA889188LA01HU diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate All lanes: HECW2 antibody at 3.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 176, 136 kDa Observed band size: 176 kDa