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Antikörper

AS3MT Antibody

Produktgrößen
50 ug
£186,00
CSB-PA888021LA01HU-50UG
100 ug
£334,00
CSB-PA888021LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA888021LA01HU
Zusätzliche Namen:
2310045H08Rik antibody; Arsenic (+3 oxidation state) methyltransferase antibody; Arsenite methyltransferase antibody; As3mt antibody; AS3MT_HUMAN antibody; C10ORF32 antibody; CYT19 antibody; Cyt19 protein antibody; Hypothetical protein C10orf32 antibody; Methylarsonite methyltransferase antibody; Methyltransferase cyt19 antibody; OTTHUMP00000020384 antibody; RP11-753C18.6 antibody; S adenosylmethionine arsenic (III) methyltransferase antibody; S-adenosyl-L-methionine:arsenic(III) methyltransferase antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. It methylates arsenite to form methylarsonate, Me-AsO(3)H(2), which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2. Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me(2)As(O)-OH (By similarity).
Host:
Rabbit
Immunogen:
Recombinant Human Arsenite methyltransferase protein (262-375AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 cells with CSB-PA888021LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA888021LA01HU diluted at 1:400 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.