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Antikörper

LYAR Antibody

Produktgrößen
50 ug
£186,00
CSB-PA882130LA01HU-50UG
100 ug
£334,00
CSB-PA882130LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA882130LA01HU
Zusätzliche Namen:
Cell growth regulating nucleolar protein antibody; Cell growth-regulating nucleolar protein antibody; Likely ortholog of mouse Ly1 antibody reactive clone antibody; Ly1 antibody reactive antibody; Ly1 antibody reactive homolog (mouse) antibody; Ly1 antibody reactive homolog antibody; LYAR antibody; LYAR_HUMAN antibody; ZC2HC2 antibody; ZLYAR antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Klonalität:
Polyclonal
Weitere Details:
nucleolus, nucleus, poly(A) RNA binding
Host:
Rabbit
Immunogen:
Recombinant Human Cell growth-regulating nucleolar protein (1-379AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA882130LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA882130LA01HU diluted at 1:500 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, PC-3 whole cell lysate All lanes: LYAR antibody at 2.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa