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Antikörper

CACYBP Antibody

Produktgrößen
50 ug
£186,00
CSB-PA872536LA01HU-50UG
100 ug
£334,00
CSB-PA872536LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA872536LA01HU
Zusätzliche Namen:
CacyBP antibody; Calcyclin binding protein antibody; Calcyclin-binding protein antibody; CYBP_HUMAN antibody; GIG 5 antibody; GIG5 antibody; Growth inhibiting gene 5 antibody; Growth inhibiting gene 5 protein antibody; hCacyBP antibody; MGC87971 antibody; PNAS 107 antibody; PNAS107 antibody; RP1 102G20.6 antibody; S100A6 binding protein antibody; S100A6-binding protein antibody; S100A6BP antibody; Siah-interacting protein antibody; SIP antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
Host:
Rabbit
Immunogen:
Recombinant Human Calcyclin-binding protein (2-228AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA872536LA01HU at dilution of 1:100
Western Blot Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HUVEC whole cell lysate All lanes: CACYBP antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 27, 9, 22 kDa Observed band size: 27 kDa
Immunofluorescence staining of U251 cells with CSB-PA872536LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating CACYBP in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA872536LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA872536LA01HU (8ug) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (10ug)