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Antikörper

NLN Antibody

Produktgrößen
50 ug
£186,00
CSB-PA863161LA01HU-50UG
100 ug
£334,00
CSB-PA863161LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA863161LA01HU
Zusätzliche Namen:
AGTBP antibody; Angiotensin binding protein antibody; Angiotensin-binding protein antibody; DKFZp564F123 antibody; EC 3.4.24.16 antibody; EP24.16 antibody; FLJ23002 antibody; KIAA1226 antibody; MEP antibody; Microsomal endopeptidase antibody; mitochondrial antibody; Mitochondrial oligopeptidase M antibody; MOP antibody; NEUL_HUMAN antibody; neurolysin (metallopeptidase M3 family) antibody; Neurolysin antibody; Neurolysin, mitochondrial precursor antibody; Neurotensin endopeptidase antibody; Nln antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A.
Host:
Rabbit
Immunogen:
Recombinant Human Neurolysin, mitochondrial protein (109-241AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 cells with CSB-PA863161LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA863161LA01HU diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: 293 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, LO2 whole cell lysate, A549 whole cell lysate All lanes: NLN antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81 kDa Observed band size: 81 kDa