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Antikörper

PARD6B Antibody

Produktgrößen
50 ul
£186,00
CSB-PA861174DSR1HU-50UL
100 ul
£334,00
CSB-PA861174DSR1HU-100UL
Über dieses Produkt
SKU:
CSB-PA861174DSR1HU
Zusätzliche Namen:
PARD6B antibody; PAR6BPartitioning defective 6 homolog beta antibody; PAR-6 beta antibody; PAR-6B antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
PBS
Klonalität:
Polyclonal
Weitere Details:
Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
Host:
Rabbit
Immunogen:
Recombinant Human Partitioning defective 6 homolog beta protein (253-372AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA861174DSR1HU at dilution of 1:100
Immunofluorescence staining of HepG2 cells with CSB-PA861174DSR1HU at 1:108, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA861174DSR1HU diluted at 1:325 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.