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Antikörper

ARID3A Antibody

Produktgrößen
50 ug
£186,00
CSB-PA858728LA01HU-50UG
100 ug
£334,00
CSB-PA858728LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA858728LA01HU
Zusätzliche Namen:
ARI3A_HUMAN antibody; ARID domain containing 3A antibody; ARID domain-containing protein 3A antibody; ARID3A antibody; AT rich interactive domain 3A (BRIGHT- like) protein antibody; AT rich interactive domain 3A (BRIGHT-like) antibody; AT rich interactive domain-containing protein 3A antibody; AT-rich interactive domain-containing protein 3A antibody; B cell regulator of IgH transcription antibody; B-cell regulator of IgH transcription antibody; Bright antibody; dead ringer like 1 antibody; dead ringer; Drosophila; homolog-like 1 antibody; Dead ringer-like protein 1 antibody; DRIL1 antibody; DRIL3 antibody; DRX antibody; E2F binding protein 1 antibody; E2F-binding protein 1 antibody; E2FBP1 antibody; Homo sapiens AT rich interactive domain 3A (BRIGHT-like) antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.
Host:
Rabbit
Immunogen:
Recombinant Human AT-rich interactive domain-containing protein 3A protein (26-137AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human, Mouse
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 cells with CSB-PA858728LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA858728LA01HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA858728LA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Mouse liver tissue All lanes: ARID3A antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 63 kDa Observed band size: 63 kDa