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Antikörper

ALG11 Antibody

Produktgrößen
50 ug
£186,00
CSB-PA647192LA01HU-50UG
100 ug
£334,00
CSB-PA647192LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA647192LA01HU
Zusätzliche Namen:
AI849156 antibody; alg11 antibody; ALG11_HUMAN antibody; Asparagine-linked glycosylation 11 antibody; Asparagine-linked glycosylation 11; alpha-1,2-mannosyltransferase homolog (yeast) antibody; Asparagine-linked glycosylation protein 11 homolog antibody; AW492253 antibody; B230397C21 antibody; GT8 antibody; KIAA0266 antibody; OTTMUSP00000022781 antibody; RGD1564725 antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
Mannosyltransferase involved in the last steps of the synthesis of Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum. Catalyzes the addition of the 4th and 5th mannose residues to the dolichol-linked oligosaccharide chain.
Host:
Rabbit
Immunogen:
Recombinant Human GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase protein (416-480AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 cells with CSB-PA647192LA01HU at 1:105, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA647192LA01HU diluted at 1:250 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.