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Antikörper

KIF22 Antibody

Produktgrößen
50 ug
£186,00
CSB-PA619894LA01HU-50UG
100 ug
£334,00
CSB-PA619894LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA619894LA01HU
Zusätzliche Namen:
A 328A3.2 antibody; KID antibody; KIF 22 antibody; kif22 antibody; KIF22_HUMAN antibody; Kinesin family member 22 antibody; Kinesin like 4 antibody; Kinesin like DNA binding protein antibody; Kinesin like DNA binding protein pseudogene antibody; Kinesin like protein 4 antibody; Kinesin like protein KIF22 antibody; Kinesin-like DNA-binding protein antibody; Kinesin-like protein 4 antibody; Kinesin-like protein KIF22 antibody; KNSL 4 antibody; KNSL4 antibody; OBP 1 antibody; OBP 2 antibody; OBP antibody; OBP1 antibody; OBP2 antibody; Origin of plasmid DNA replication binding protein antibody; OriP binding protein antibody; OTTHUMP00000123406 antibody; SEMDJL2 antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA (By similarity). Plays a role in congression of laterally attached chromosomes in NDC80-depleted cells (PubMed:25743205).
Host:
Rabbit
Immunogen:
Recombinant Human Kinesin-like protein KIF22 protein (34-169AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of NIH/3T3 cells with CSB-PA619894LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA619894LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.