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Antikörper

ASIC2 Antibody

Produktgrößen
50 ug
£186,00
CSB-PA613683LA01HU-50UG
100 ug
£334,00
CSB-PA613683LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA613683LA01HU
Zusätzliche Namen:
ACCN antibody; ACCN1 antibody; ACCN1_HUMAN antibody; Acid sensing ion channel 2 antibody; Acid-sensing ion channel 2 antibody; Amiloride sensitive brain sodium channel antibody; Amiloride sensitive cation channel 1 neuronal (degenerin) antibody; Amiloride sensitive cation channel 1 neuronal antibody; Amiloride-sensitive brain sodium channel antibody; Amiloride-sensitive cation channel 1; neuronal antibody; Amiloride-sensitive cation channel neuronal 1 antibody; ASIC2 antibody; ASIC2a antibody; BNaC1 antibody; BNC1 antibody; Brain sodium channel 1 antibody; Degenerin antibody; hBNaC1 antibody; Mammalian degenerin homolog antibody; MDEG antibody; Neuronal amiloride sensitive cation channel 1 antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate.
Host:
Rabbit
Immunogen:
Recombinant Human Acid-sensing ion channel 2 protein (197-345AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 cells with CSB-PA613683LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA613683LA01HU diluted at 1:300 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA613683LA01HU diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.