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Antikörper

PDE8A Antibody

Produktgrößen
50 ul
£186,00
CSB-PA525315LA01HU-50UL
100 ul
£334,00
CSB-PA525315LA01HU-100UL
Über dieses Produkt
SKU:
CSB-PA525315LA01HU
Zusätzliche Namen:
5''-cyclic phosphodiesterase 8A antibody; cAMP specific cyclic nucleotide phosphodiesterase 8A antibody; FLJ16150 antibody; High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A antibody; High affinity cAMP-specific and IBMX-insensitive 3'' antibody; HsT19550 antibody; PDE 8A antibody; PDE8A antibody; PDE8A_HUMAN antibody; Phosphodiesterase 8A antibody; Phosphodiesterase8A antibody; Weakly similar to 3'5' cyclic nucleotide phosphodiesterase antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed:18983167). May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development (PubMed:18983167). Binding to RAF1 reduces RAF1 'Ser-259' inhibitory-phosphorylation and stimulates RAF1-dependent EGF-activated ERK-signaling (PubMed:23509299). Protects against cell death induced by hydrogen peroxide and staurosporine (PubMed:23509299).
Host:
Rabbit
Immunogen:
Recombinant Human High affinity cAMP-specific and IBMX-insensitive 3\',5\'-cyclic phosphodiesterase 8A protein (596-829AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 cells with CSB-PA525315LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA525315LA01HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.