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Antikörper

GLI1 Antibody

Produktgrößen
50 ug
£186,00
CSB-PA12989A0RB-50UG
100 ug
£334,00
CSB-PA12989A0RB-100UG
Über dieses Produkt
SKU:
CSB-PA12989A0RB
Zusätzliche Namen:
Gli 1 antibody; GLI antibody; GLI family zinc finger 1 antibody; GLI Kruppel family member 1 antibody; gli1 antibody; GLI1_HUMAN antibody; Glioma associated oncogene 1 antibody; Glioma associated oncogene homolog 1 (zinc finger protein) antibody; Glioma associated oncogene homolog antibody; Glioma-associated oncogene antibody; Oncogene GLI antibody; Zfp 5 antibody; Zfp5 antibody; Zinc finger protein GLI 1 antibody; Zinc finger protein GLI1 antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Klonalität:
Polyclonal
Weitere Details:
Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
Host:
Rabbit
Immunogen:
Recombinant Human Zinc finger protein GLI1 protein (921-1106AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 cells with CSB-PA12989A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA12989A0Rb diluted at 1:400 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.