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Antikörper

AQP4 Antibody

Produktgrößen
50 ug
£186,00
CSB-PA11137A0RB-50UG
100 ug
£334,00
CSB-PA11137A0RB-100UG
Über dieses Produkt
SKU:
CSB-PA11137A0RB
Zusätzliche Namen:
AQP 4 antibody; AQP-4 antibody; AQP4 antibody; AQP4_HUMAN antibody; Aquaporin type 4 antibody; Aquaporin-4 antibody; Aquaporin4 antibody; HMIWC 2 antibody; HMIWC2 antibody; Mercurial insensitive water channel antibody; Mercurial-insensitive water channel antibody; MGC22454 antibody; MIWC antibody; WCH 4 antibody; WCH4 antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Klonalität:
Polyclonal
Weitere Details:
Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
Host:
Rabbit
Immunogen:
Recombinant Human Aquaporin-4 protein (253-323AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human, Mouse
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Western blot All lanes: AQP4 antibody at 2ug/ml Lane 1: Mouse heart tissue Lane 2: Mouse brain tissue Lane 3: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 33 kDa Observed band size: 35 kDa
Immunofluorescence staining of U251 cells with CSB-PA11137A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA11137A0Rb diluted at 1:370 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA11137A0Rb diluted at 1:370 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.