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Antikörper

CSNK2A3 Antibody

Produktgrößen
50 ul
£186,00
CSB-PA086427LA01HU-50UL
100 ul
£334,00
CSB-PA086427LA01HU-100UL
Über dieses Produkt
SKU:
CSB-PA086427LA01HU
Zusätzliche Namen:
CSNK2A3 antibody; CSNK2A1PCasein kinase II subunit alpha 3 antibody; CK II alpha 3 antibody; EC 2.7.11.1 antibody; Casein kinase II alpha 1 polypeptide pseudogene antibody
Anwendung:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
Probable catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Amplification-dependent oncogene; promotes cell proliferation and tumorigenesis by down-regulating expression of the tumor suppressor protein, PML. May play a role in the pathogenesis of the lung cancer development and progression.
Host:
Rabbit
Immunogen:
Recombinant Human Casein kinase II subunit alpha 3 protein (151-391AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
IHC image of CSB-PA086427LA01HU diluted at 1:400 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating CSNK2A3 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA086427LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000) Lane 2: CSB-PA086427LA01HU (5ug) + Hela whole cell lysate (0.5mg) Lane 3: Hela whole cell lysate (20ug)
Western Blot Positive WB detected in: PC3 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, THP-1 whole cell lysate All lanes: CSNK2A3 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa