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Antikörper

EZR Antibody

Produktgrößen
50 ug
£186,00
CSB-PA02159A0RB-50UG
100 ug
£334,00
CSB-PA02159A0RB-100UG
Über dieses Produkt
SKU:
CSB-PA02159A0RB
Zusätzliche Namen:
Villin 2 ezrin antibody; CVIL antibody; CVL antibody; Cytovillin 2 antibody; Cytovillin antibody; DKFZp762H157 antibody; Epididymis secretory protein Li 105 antibody; EZR antibody; EZRI_HUMAN antibody; Ezrin antibody; FLJ26216 antibody; HEL S 105 antibody; MGC1584 antibody; p81 antibody; VIL 2 antibody; VIL2 antibody; Villin 2 (ezrin) antibody; Villin 2 antibody; Villin-2 antibody; Villin2 antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Klonalität:
Polyclonal
Weitere Details:
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Host:
Rabbit
Immunogen:
Recombinant Human Ezrin protein (1-251AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA02159A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA02159A0Rb diluted at 1:300 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA02159A0Rb diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.