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Antikörper

NEFH Antibody

Produktgrößen
50 ul
£186,00
CSB-PA015686LA01HU-50UL
100 ul
£334,00
CSB-PA015686LA01HU-100UL
Über dieses Produkt
SKU:
CSB-PA015686LA01HU
Zusätzliche Namen:
200 kDa neurofilament protein antibody; CMT2CC antibody; Nefh antibody; Neurofilament heavy polypeptide 200kDa antibody; Neurofilament heavy polypeptide antibody; Neurofilament triplet H protein antibody; NF H antibody; NF-H antibody; NFH antibody; NFH_HUMAN antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Klonalität:
Polyclonal
Weitere Details:
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Host:
Rabbit
Immunogen:
Recombinant Human Neurofilament heavy polypeptide protein (825-1003AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of SH-SY5Y cells with CSB-PA015686LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA015686LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.