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Antikörper

MDH1 Antibody

Produktgrößen
50 ug
£186,00
CSB-PA013621EA01HU-50UG
100 ug
£334,00
CSB-PA013621EA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA013621EA01HU
Zusätzliche Namen:
cytoplasmic antibody; Cytosolic malate dehydrogenase antibody; Diiodophenylpyruvate reductase antibody; Malate dehydrogenase 1, NAD (soluble) antibody; Malate dehydrogenase antibody; Malate dehydrogenase cytoplasmic antibody; MDH s antibody; mdh1 antibody; MDHA antibody; MDHC_HUMAN antibody; MDHs antibody; MGC:1375 antibody; MOR2 antibody; Soluble malate dehydrogenase antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
cytosol, extracellular exosome, extracellular space, mitochondrion, L-malate dehydrogenase activity, malic enzyme activity, gluconeogenesis, malate metabolic process, NADH metabolic process, oxaloacetate metabolic process
Host:
Rabbit
Immunogen:
Recombinant Human Malate dehydrogenase, cytoplasmic protein (1-334AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human, Mouse
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of U251 cells with CSB-PA013621EA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA013621EA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: HL60 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, LO2 whole cell lysate, NIH/3T3 whole cell lysate, RAW264.7 whole cell lysate, Mouse liver tissue All lanes: MDH1 antibody at 2.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37, 28, 39 kDa Observed band size: 37 kDa