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Antikörper

MAGOH Antibody

Produktgrößen
50 ul
£186,00
CSB-PA013364ESR1HU-50UL
100 ul
£334,00
CSB-PA013364ESR1HU-100UL
Über dieses Produkt
SKU:
CSB-PA013364ESR1HU
Zusätzliche Namen:
Mago nashi homolog proliferation associated (Drosophila) antibody; Mago nashi protein homolog antibody; magoh antibody; MAGOHA antibody; MGN_HUMAN antibody; Protein mago nashi homolog antibody
Anwendung:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
Klonalität:
Polyclonal
Weitere Details:
Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.
Host:
Rabbit
Immunogen:
Recombinant Human Protein mago nashi homolog protein (1-146AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human, Mouse
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA013364ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA013364ESR1HU at dilution of 1:100
Western blot All lanes: MAGOH antibody at 4.69ug/ml Lane 1: Mouse kidney tissue Lane 2: A431 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Raji whole cell lysate Lane 5: K562 whole cell lysate Lane 6: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 18, 13 kDa Observed band size: 18 kDa
Immunoprecipitating MAGOH in Hela whole cell lysate Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA013364ESR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: CSB-PA013364ESR1HU (4ug) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (20ug)