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Antikörper

CSNK1E Antibody

Produktgrößen
50 ug
£186,00
CSB-PA01319A0RB-50UG
100 ug
£334,00
CSB-PA01319A0RB-100UG
Über dieses Produkt
SKU:
CSB-PA01319A0RB
Zusätzliche Namen:
Casein kinase 1 epsilon antibody; Casein kinase I antibody; Casein kinase I epsilon antibody; Casein kinase I isoform epsilon antibody; CKI epsilon antibody; CKI-epsilon antibody; CKIe antibody; CKIepsilon antibody; CSNK 1E antibody; CSNK1E antibody; DBT antibody; Doubletime, Drosophila, homolog of antibody; EC 2.7.11.1 antibody; epsilon isoform antibody; HCK IE antibody; KC1E antibody; KC1E_HUMAN antibody; kinase CK1 epsilon antibody; MGC 10398 antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Klonalität:
Polyclonal
Weitere Details:
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phospohorylation. Controls PER1 and PER2 nuclear transport and degradation. Inhibits cytokine-induced granuloytic differentiation.
Host:
Rabbit
Immunogen:
Recombinant Human Casein kinase I isoform epsilon protein (1-416AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA01319A0Rb at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA01319A0Rb diluted at 1:800 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: CSNK1E antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48 kDa Observed band size: 48 kDa