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Antikörper

H1F0 (Ab-101) Antibody

Produktgrößen
50 ul
£195,00
CSB-PA010087OA101NME1HU-50UL
100 ul
£356,00
CSB-PA010087OA101NME1HU-100UL
Über dieses Produkt
SKU:
CSB-PA010087OA101NME1HU
Zusätzliche Namen:
H1 histone family member 0 antibody; H1(0) antibody; H10 antibody; H10_HUMAN antibody; h1f0 antibody; H1FV antibody; Histone H1'' antibody; Histone H1(0) antibody; Histone H1.0 antibody; Histone H10 antibody; Histone H5 antibody; MGC5241 antibody; N-terminally processed antibody
Anwendung:
ChIP, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
Host:
Rabbit
Immunogen:
Peptide sequence around site of Lys (101) derived from Human Histone H1.0
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human, Rat
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-H1F0 (CSB-PA010087OA101nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the B Beta-Globin promoter.
Immunofluorescence staining of HepG2 cells with CSB-PA010087OA101nme1HU at 1:1, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA010087OA101nme1HU diluted at 1:50 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Rat spleen tissue All lanes: H1F0 antibody at 0.85ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21, 20 kDa Observed band size: 21 kDa