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Antikörper

FLOT1 Antibody

Produktgrößen
50 ug
£186,00
CSB-PA008727LA01HU-50UG
100 ug
£334,00
CSB-PA008727LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA008727LA01HU
Zusätzliche Namen:
FLOT 1 antibody; FLOT1 antibody; FLOT1_HUMAN antibody; Flotillin-1 antibody; Flotillin1 antibody; Integral membrane component of caveolae antibody; Reggie 2 antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Host:
Rabbit
Immunogen:
Recombinant Human Flotillin-1 protein (169-251AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human, Mouse, Rat
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
IHC image of CSB-PA008727LA01HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA008727LA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated ABC system.
Western Blot Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Rat brain tissue, Mouse brain tissue All lanes: FLOT1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48, 43 kDa Observed band size: 48 kDa
Immunofluorescence staining of Hela cells with CSB-PA008727LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating FLOT1 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA008727LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA008727LA01HU (8ug) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (10ug)