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Antikörper

ENTPD1 Antibody

Produktgrößen
50 ug
£186,00
CSB-PA007690LA01HU-50UG
100 ug
£334,00
CSB-PA007690LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA007690LA01HU
Zusätzliche Namen:
ATPDase antibody; CD 39 antibody; CD39 antibody; CD39 antigen antibody; DKFZp686D194 antibody; DKFZp686I093 antibody; Ecto apyrase antibody; Ecto ATP diphosphohydrolase antibody; Ecto-apyrase antibody; Ecto-ATP diphosphohydrolase 1 antibody; Ecto-ATPase 1 antibody; Ecto-ATPDase 1 antibody; Ectonucleoside triphosphate diphosphohydrolase 1 antibody; ENTP1_HUMAN antibody; ENTPD 1 antibody; ENTPD1 antibody; FLJ40921 antibody; FLJ40959 antibody; Lymphoid cell activation antigen antibody; NTPDase 1 antibody; NTPDase1 antibody; SPG64 antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well.
Host:
Rabbit
Immunogen:
Recombinant Human Ectonucleoside triphosphate diphosphohydrolase 1 protein (316-474AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA007690LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA007690LA01HU diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: HL-60 whole cell lysate, HepG2 whole cell lysate, U251 whole cell lysate All lanes: ENTPD1 antibody at 2.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58, 59, 35, 43, 47, 60 kDa Observed band size: 58, 43 kDa