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Antikörper

CHRNB2 Antibody

Produktgrößen
50 ug
£186,00
CSB-PA005396LA01HU-50UG
100 ug
£334,00
CSB-PA005396LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA005396LA01HU
Zusätzliche Namen:
Acetylcholine receptor beta 2 neural antibody; ACHB2_HUMAN antibody; ACHN antibody; AChR antibody; Acrb 2 antibody; Acrb2 antibody; b2 nAchR antibody; Cholinergic receptor nicotinic beta 2 antibody; Cholinergic receptor nicotinic beta polypeptide 2 antibody; Cholinergic receptor nicotinic beta polypeptide 2 neuronal antibody; cholinergic receptor; nicotinic; beta 2 (neuronal) antibody; Chrnb2 antibody; EFNL 3 antibody; EFNL3 antibody; nAChRB2 antibody; Neuronal acetylcholine receptor protein beta 2 chain precursor antibody; Neuronal acetylcholine receptor protein subunit beta 2 antibody; Neuronal acetylcholine receptor subunit beta-2 antibody; Neuronal nicotinic acetylcholine receptor beta 2 antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodiun ions.
Host:
Rabbit
Immunogen:
Recombinant Human Neuronal acetylcholine receptor subunit beta-2 protein (322-458AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human, Rat
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of SH-SY5Y cells with CSB-PA005396LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA005396LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: CHRNB2 antibody at 6.05ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa