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Antikörper

CCKAR Antibody

Produktgrößen
50 ug
£186,00
CSB-PA004772LA01HU-50UG
100 ug
£334,00
CSB-PA004772LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA004772LA01HU
Zusätzliche Namen:
CCK A antibody; CCK A receptor antibody; CCK AR antibody; CCK-A receptor antibody; CCK-AR antibody; CCK1 R antibody; CCK1-R antibody; CCK1R antibody; CCKA antibody; CCKA receptor antibody; CCKAR antibody; CCKAR_HUMAN antibody; CCKR Type A antibody; CCKRA antibody; Cholecystokinin 1 receptor antibody; Cholecystokinin A receptor antibody; Cholecystokinin receptor type A antibody; Cholecystokinin type A receptor antibody; Cholecystokinin-1 receptor antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Host:
Rabbit
Immunogen:
Recombinant Human Cholecystokinin receptor type A protein (235-313AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA004772LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA004772LA01HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: A549 whole cell lysate All lanes: CCKAR antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48 kDa Observed band size: 48 kDa