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Antikörper

CA4 Antibody

Produktgrößen
50 ug
£186,00
CSB-PA004372LA01HU-50UG
100 ug
£334,00
CSB-PA004372LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA004372LA01HU
Zusätzliche Namen:
CA IV antibody; CA4 antibody; CAH4_HUMAN antibody; CAIV antibody; Car4 antibody; Carbonate dehydratase IV antibody; Carbonic anhydrase 4 antibody; Carbonic dehydratase antibody; Carbonic dehydratase IV antibody; EC 4.2.1.1 antibody; Retinitis pigmentosa 17 (autosomal dominant) antibody; RP17 antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.
Host:
Rabbit
Immunogen:
Recombinant Human Carbonic anhydrase 4 protein (75-200AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
IHC image of CSB-PA004372LA01HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CSB-PA004372LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).