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Antikörper

ATP7A Antibody

Produktgrößen
50 ug
£186,00
CSB-PA002414LA01HU-50UG
100 ug
£334,00
CSB-PA002414LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA002414LA01HU
Zusätzliche Namen:
ATP 7A antibody; ATP7A antibody; ATP7A_HUMAN antibody; ATPase copper transporting alpha polypeptide antibody; ATPase Cu++ transporting alpha polypeptide (Menkes syndrome) antibody; ATPase Cu++ transporting alpha polypeptide antibody; Copper pump 1 antibody; Copper transporting ATPase 1 antibody; Copper-transporting ATPase 1 antibody; Cu++ transporting P type ATPase antibody; DSMAX antibody; FLJ17790 antibody; MC 1 antibody; MC1 antibody; Menkes disease associated protein antibody; Menkes disease-associated protein antibody; Menkes syndrome antibody; MK antibody; MNK antibody; OHS antibody; OTTHUMP00000062077 antibody; SMAX3 antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
May supply copper to copper-requiring proteins within the secretory pathway, when localized in the trans-Golgi network. Under conditions of elevated extracellular copper, it relocalized to the plasma membrane where it functions in the efflux of copper from cells.
Host:
Rabbit
Immunogen:
Recombinant Human Copper-transporting ATPase 1 protein (257-488AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA002414LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA863972LA01HU diluted at 1: 500 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.