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Antikörper

ALDH7A1 Antibody

Produktgrößen
50 ug
£186,00
CSB-PA001579LA01HU-50UG
100 ug
£334,00
CSB-PA001579LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA001579LA01HU
Zusätzliche Namen:
26g turgor protein homolog antibody; AL7A1_HUMAN antibody; Aldehyde dehydrogenase 7 A1 antibody; Aldehyde dehydrogenase 7 family, member A1 antibody; Aldehyde dehydrogenase family 7 member A1 antibody; ALDH7A1 antibody; Alpha AASA dehydrogenase antibody; Alpha aminoadipic semialdehyde dehydrogenase antibody; Alpha-AASA dehydrogenase antibody; Alpha-aminoadipic semialdehyde dehydrogenase antibody; Antiquitin 1 antibody; Antiquitin antibody; Antiquitin-1 antibody; ATQ1 antibody; Betaine aldehyde dehydrogenase antibody; Delta1 piperideine 6 carboxylate dehydrogenease antibody; Delta1-piperideine-6-carboxylate dehydrogenase antibody; EPD antibody; P6c dehydrogenase antibody; PDE antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism.
Host:
Rabbit
Immunogen:
Recombinant Human Alpha-aminoadipic semialdehyde dehydrogenase protein (386-515AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human, Mouse, Rat
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
Immunofluorescence staining of Hela cells with CSB-PA001579LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, SH-SY5Y whole cell lysate, Rat liver tissue, Rat kidney tissue, Mouse liver tissue, Mouse kidney tissue All lanes: ALDH7A1 antibody at 4.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59, 56, 52 kDa Observed band size: 59 kDa
IHC image of CSB-PA001579LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA001579LA01HU diluted at 1:400 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating ALDH7A1 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA001579LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA001579LA01HU (6ug) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (20ug)