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Antikörper

ADH1A Antibody

Produktgrößen
50 ug
£186,00
CSB-PA001353LA01HU-50UG
100 ug
£334,00
CSB-PA001353LA01HU-100UG
Über dieses Produkt
SKU:
CSB-PA001353LA01HU
Zusätzliche Namen:
ADH alpha subunit antibody; ADH antibody; ADH1 antibody; ADH1A antibody; ADH1A_HUMAN antibody; Alcohol dehydrogenase 1 (class I), alpha polypeptide antibody; Alcohol dehydrogenase 1 antibody; Alcohol dehydrogenase 1A (class I), alpha polypeptide antibody; Alcohol dehydrogenase 1A antibody; Alcohol dehydrogenase subunit alpha antibody; Aldehyde reductase antibody
Anwendung:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Klonalität:
Polyclonal
Weitere Details:
cytosol, nucleoplasm, plasma membrane, alcohol dehydrogenase (NAD) activity, alcohol dehydrogenase activity, zinc-dependent, retinol dehydrogenase activity, alcohol metabolic process, drug metabolic process, ethanol oxidation
Host:
Rabbit
Immunogen:
Recombinant Human Alcohol dehydrogenase 1A protein (104-253AA)
Isotyp:
IgG
Physischer Zustand:
Liquid
Aufreinigung:
Protein G Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Hersteller:
Cusabio
Typ:
Antibody: Polyclonal Antibody
IHC image of CSB-PA001353LA01HU diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA001353LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CSB-PA001353LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).